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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
24/11/2010 |
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Data da última atualização: |
04/04/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUSA, T. N.; TARAZONA-SANTOS, E. M.; WILSON, D. J.; MADUREIRA, A. P.; FALCAO, P. R. K.; FONTES, C. J. F.; GIL, L. H. S.; FERREIRA, M. U.; CARVALHO, L. H.; BRITO, C. F. A. |
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Afiliação: |
TAIS N. SOUSA, CPqRR/FIOCRUZ; EDUARDO M. TARAZONA-SANTOS, ICB/UFMG; DANIEL J. WILSON, University of Chicago; ANA P. MADUREIRA, Universidade Federal de São João del Rei; PAULA REGINA KUSER FALCAO, CNPTIA; COR J. F. FONTES, UFMT; LUIZ H. S. GIL, IPEPATRO; MARCELO U. FERREIRA, USP; LUZIA H. CARVALHO, CPqRR/FIOCRUZ; CRISTIANA F. A. BRITO, CPqRR/FIOCRUZ. |
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Título: |
Genetic variability and natural selection at the ligand domain of the Duffy binding protein in Brazilian Plasmodium vivax populations. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Malaria Journal, London, v. 9, n. 334, 2010. |
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Páginas: |
12 p. |
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DOI: |
doi:10.1186/1475-2875-9-334 |
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Idioma: |
Inglês |
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Conteúdo: |
Background. Plasmodium vivax malaria is a major public health challenge in Latin America, Asia and Oceania, with 130-435 million clinical cases per year worldwide. Invasion of host blood cells by P. vivax mainly depends on a type I membrane protein called Duffy binding protein (PvDBP). The erythrocyte-binding motif of PvDBP is a 170 amino-acid stretch located in its cysteine-rich region II (PvDBPII), which is the most variable segment of the protein. Methods. To test whether diversifying natural selection has shaped the nucleotide diversity of PvDBPII in Brazilian populations, this region was sequenced in 122 isolates from six different geographic areas. A Bayesian method was applied to test for the action of natural selection under a population genetic model that incorporates recombination. The analysis was integrated with a structural model of PvDBPII, and T- and B-cell epitopes were localized on the 3-D structure. Results. The results suggest that: (i) recombination plays an important role in determining the haplotype structure of PvDBPII, and (ii) PvDBPII appears to contain neutrally evolving codons as well as codons evolving under natural selection. Diversifying selection preferentially acts on sites identified as epitopes, particularly on amino acid residues 417, 419, and 424, which show strong linkage disequilibrium. Conclusions. This study shows that some polymorphisms of PvDBPII are present near the erythrocyte-binding domain and might serve to elude antibodies that inhibit cell invasion. Therefore, these polymorphisms should be taken into account when designing vaccines aimed at eliciting antibodies to inhibit erythrocyte invasion. MenosBackground. Plasmodium vivax malaria is a major public health challenge in Latin America, Asia and Oceania, with 130-435 million clinical cases per year worldwide. Invasion of host blood cells by P. vivax mainly depends on a type I membrane protein called Duffy binding protein (PvDBP). The erythrocyte-binding motif of PvDBP is a 170 amino-acid stretch located in its cysteine-rich region II (PvDBPII), which is the most variable segment of the protein. Methods. To test whether diversifying natural selection has shaped the nucleotide diversity of PvDBPII in Brazilian populations, this region was sequenced in 122 isolates from six different geographic areas. A Bayesian method was applied to test for the action of natural selection under a population genetic model that incorporates recombination. The analysis was integrated with a structural model of PvDBPII, and T- and B-cell epitopes were localized on the 3-D structure. Results. The results suggest that: (i) recombination plays an important role in determining the haplotype structure of PvDBPII, and (ii) PvDBPII appears to contain neutrally evolving codons as well as codons evolving under natural selection. Diversifying selection preferentially acts on sites identified as epitopes, particularly on amino acid residues 417, 419, and 424, which show strong linkage disequilibrium. Conclusions. This study shows that some polymorphisms of PvDBPII are present near the erythrocyte-binding domain and might serve to elude antibodies that... Mostrar Tudo |
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Palavras-Chave: |
Genetic variability; Modelagem estrutural; Seleção natural; Structural modelling; Variabilidade genética. |
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Thesagro: |
Polimorfismo. |
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Thesaurus Nal: |
malaria; Natural selection; Plasmodium vivax; Polymorphism. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/80555/1/1475-2875-9-334-4.pdf
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Marc: |
LEADER 02791naa a2200373 a 4500
001 1867894
005 2013-04-04
008 2010 bl uuuu u00u1 u #d
024 7 $adoi:10.1186/1475-2875-9-334$2DOI
100 1 $aSOUSA, T. N.
245 $aGenetic variability and natural selection at the ligand domain of the Duffy binding protein in Brazilian Plasmodium vivax populations.$h[electronic resource]
260 $c2010
300 $a12 p.
520 $aBackground. Plasmodium vivax malaria is a major public health challenge in Latin America, Asia and Oceania, with 130-435 million clinical cases per year worldwide. Invasion of host blood cells by P. vivax mainly depends on a type I membrane protein called Duffy binding protein (PvDBP). The erythrocyte-binding motif of PvDBP is a 170 amino-acid stretch located in its cysteine-rich region II (PvDBPII), which is the most variable segment of the protein. Methods. To test whether diversifying natural selection has shaped the nucleotide diversity of PvDBPII in Brazilian populations, this region was sequenced in 122 isolates from six different geographic areas. A Bayesian method was applied to test for the action of natural selection under a population genetic model that incorporates recombination. The analysis was integrated with a structural model of PvDBPII, and T- and B-cell epitopes were localized on the 3-D structure. Results. The results suggest that: (i) recombination plays an important role in determining the haplotype structure of PvDBPII, and (ii) PvDBPII appears to contain neutrally evolving codons as well as codons evolving under natural selection. Diversifying selection preferentially acts on sites identified as epitopes, particularly on amino acid residues 417, 419, and 424, which show strong linkage disequilibrium. Conclusions. This study shows that some polymorphisms of PvDBPII are present near the erythrocyte-binding domain and might serve to elude antibodies that inhibit cell invasion. Therefore, these polymorphisms should be taken into account when designing vaccines aimed at eliciting antibodies to inhibit erythrocyte invasion.
650 $amalaria
650 $aNatural selection
650 $aPlasmodium vivax
650 $aPolymorphism
650 $aPolimorfismo
653 $aGenetic variability
653 $aModelagem estrutural
653 $aSeleção natural
653 $aStructural modelling
653 $aVariabilidade genética
700 1 $aTARAZONA-SANTOS, E. M.
700 1 $aWILSON, D. J.
700 1 $aMADUREIRA, A. P.
700 1 $aFALCAO, P. R. K.
700 1 $aFONTES, C. J. F.
700 1 $aGIL, L. H. S.
700 1 $aFERREIRA, M. U.
700 1 $aCARVALHO, L. H.
700 1 $aBRITO, C. F. A.
773 $tMalaria Journal, London$gv. 9, n. 334, 2010.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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| Registros recuperados : 2 | |
| 1. |  | ARAÚJO, M. S.; MESSIAS, M. R.; FIGUEIRO, M. R.; GIL, L. H. S.; PROBST, C. M.; VIDAL, N. M.; KATSURAGAWA, T. H.; KRIEGER, M. A.; SILVA, L. H. P. da; OZAKI, L. S. Natural Plasmodium infection in monkeys in the state of Rondônia (Brazilian Western Amazon). Malaria Journal, v. 12, article 180, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 2. | | SOUSA, T. N.; TARAZONA-SANTOS, E. M.; WILSON, D. J.; MADUREIRA, A. P.; FALCAO, P. R. K.; FONTES, C. J. F.; GIL, L. H. S.; FERREIRA, M. U.; CARVALHO, L. H.; BRITO, C. F. A. Genetic variability and natural selection at the ligand domain of the Duffy binding protein in Brazilian Plasmodium vivax populations. Malaria Journal, London, v. 9, n. 334, 2010. 12 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| Registros recuperados : 2 | |
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